Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection
نویسندگان
چکیده
منابع مشابه
Coronavirus avian infectious bronchitis virus.
Infectious bronchitis virus (IBV), the coronavirus of the chicken (Gallus gallus), is one of the foremost causes of economic loss within the poultry industry, affecting the performance of both meat-type and egg-laying birds. The virus replicates not only in the epithelium of upper and lower respiratory tract tissues, but also in many tissues along the alimentary tract and elsewhere e.g. kidney,...
متن کاملRecombination in Avian Gamma-Coronavirus Infectious Bronchitis Virus
Recombination in the family Coronaviridae has been well documented and is thought to be a contributing factor in the emergence and evolution of different coronaviral genotypes as well as different species of coronavirus. However, there are limited data available on the frequency and extent of recombination in coronaviruses in nature and particularly for the avian gamma-coronaviruses where only ...
متن کاملpH stability studies with avian infectious bronchitis virus (coronavirus) strains.
A comparison of 17 infectious bronchitis virus strains, using the same test procedure and assay system, demonstrated that stability at an acid pH is a variable characteristic of the avian coronaviruses.
متن کاملReverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus.
We have developed a reverse genetics system for the avian coronavirus infectious bronchitis virus (IBV) in which a full-length cDNA corresponding to the IBV genome is inserted into the vaccinia virus genome under the control of a T7 promoter sequence. Vaccinia virus as a vector for the full-length IBV cDNA has the advantage that modifications can be introduced into the IBV cDNA using homologous...
متن کاملAvian infectious bronchitis virus.
Infectious bronchitis virus (IBV) is prevalent in all countries with an intensive poultry industry, with the incidence of infection approaching 100% in most locations. Vaccination is only partially successful due to the continual emergence of antigenic variants. At many sites, multiple antigenic types are simultaneously present, requiring the application of multiple vaccines. Although many coun...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2005
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2004.09.017